Optimization of Fermentation Conditions for Taxol Production from Endophytic Fungus by Response Surface Methodology
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摘要: 选择产紫杉醇的内生真菌EF-04和葡萄糖、豆饼粉、乙酸钠三种营养因子,在单因素试验的基础上,通过Box-Benhnken组合设计和响应曲面分析法优化内生真菌液体发酵产紫杉醇的发酵工艺条件进行紫杉醇人工生产试验。响应面分析结果表明,内生真菌液体发酵产紫杉醇的最佳培养条件为葡萄糖25.54g/L、豆饼粉7.94 g/L、乙酸钠5.64 g/L;此条件下的验证试验表明,实际紫杉醇得率为313.52 μg/L,与理论值基本吻合。
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关键词:
- 紫杉醇 /
- 内生真菌 /
- 发酵 /
- Box-Behnken /
- 响应面
Abstract: Based on the results of single factor experiment, three levels and three factors of Box-Behnken design was employed combining with response surface methodology (RSM) to optimize the fermentation conditions for taxol production from endophytic fungus. The result showed that the optimal fermentation conditions were as follows: glucose 25.54 g/L, soybean powder7.94 g/L and sodium acetate 5.64g/L. Verification test demonstrated that the average yield of taxol was 313.52 μg/L, similar to theoretical value of 331.6 2μg/L.-
Keywords:
- taxol /
- endophytic fungus /
- fermentation /
- Box-Behnken /
- Response surface methodology (RSM)
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[1] Wani M C, Taylor H L, Wall M E, et al. Plant antitumor agents. VI. The isolation and structure of Taxol, a novel antileukemic and antitumor agent from Taxus brevifolia[J]. J Am Chem Soc 1971, 93(9): 2325-2327.
[2] Stierle A , Strobel G, Stierle D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of pacific yew[J]. Science, 1993,260(5105):214-216.
[3] 靳瑞,康冀川,文庭池,等. 一株产紫杉醇内生真菌液体发酵工艺的优化[J]. 菌物学报,2011(2):235-241. [4] 邱德有,黄美娟,方晓华,等. 一种云南红豆杉内生真菌的分离[J]. 真菌学报,1994,13(4):314-316. [5] 周东坡,孙剑秋,于寒颖,等. 中国一新记录属—多节孢属[J]. 菌物系统,2001,20(2):148-149. [6] 吕国英,张作法,潘慧娟,等. 响应面分析法优化香菇多糖发酵培养基[J]. 菌物学报,2010,29(1):106-112. [7] 王允祥,吕凤霞,陆兆新. 杯伞发酵培养基的响应曲面法优化研究[J]. 南京农业大学学报,2004,27(3):89-94. [8] Liang Renjie. Optimization of extraction process of Glycyrrhiza glabra polysaccharides by response surface methodology[J]. Carbohydr Polym,2008,74(4):858-861.
[9] 费荣昌. 实验设计与数据处理(第四版)[M]. 无锡:江南大学出版社,2001.
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