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响应面法优化内生真菌发酵产紫杉醇工艺

程俊文, 贺亮, 邹景泉, 方茹, 邱德有, 胡传久, 魏海龙, 蒋云鹤, 王衍彬

程俊文, 贺亮, 邹景泉, 方茹, 邱德有, 胡传久, 魏海龙, 蒋云鹤, 王衍彬. 响应面法优化内生真菌发酵产紫杉醇工艺[J]. 浙江林业科技, 2015, 35(6): 68-72.
引用本文: 程俊文, 贺亮, 邹景泉, 方茹, 邱德有, 胡传久, 魏海龙, 蒋云鹤, 王衍彬. 响应面法优化内生真菌发酵产紫杉醇工艺[J]. 浙江林业科技, 2015, 35(6): 68-72.
CHENG Jun-wen, HE Liang, ZOU Jing-quan, FANG Ru, QIU De-you, HU Chuan-jiu, WEI Hai-long, JIANG Yun-he, WANG Yan-bin. Optimization of Fermentation Conditions for Taxol Production from Endophytic Fungus by Response Surface Methodology[J]. Journal of Zhejiang Forestry Science and Technology, 2015, 35(6): 68-72.
Citation: CHENG Jun-wen, HE Liang, ZOU Jing-quan, FANG Ru, QIU De-you, HU Chuan-jiu, WEI Hai-long, JIANG Yun-he, WANG Yan-bin. Optimization of Fermentation Conditions for Taxol Production from Endophytic Fungus by Response Surface Methodology[J]. Journal of Zhejiang Forestry Science and Technology, 2015, 35(6): 68-72.

响应面法优化内生真菌发酵产紫杉醇工艺

基金项目: 浙江省省院合作林业科技项目(2013SY17);浙江省科技厅院所专项项目(2014F30001);浙江省自然科学基金项目(LQ15C160001); 国家自然科学基金项目(31170628,31300567)、中央级公益性科研院所项目(RIF2014-01)的部分研究内容
详细信息
    作者简介:

    程俊文(1981-),男,安徽省芜湖人,硕士,副研究员,从事森林食品及林源中药活性成分开发研究。

  • 中图分类号: S718.8

Optimization of Fermentation Conditions for Taxol Production from Endophytic Fungus by Response Surface Methodology

  • 摘要: 选择产紫杉醇的内生真菌EF-04和葡萄糖、豆饼粉、乙酸钠三种营养因子,在单因素试验的基础上,通过Box-Benhnken组合设计和响应曲面分析法优化内生真菌液体发酵产紫杉醇的发酵工艺条件进行紫杉醇人工生产试验。响应面分析结果表明,内生真菌液体发酵产紫杉醇的最佳培养条件为葡萄糖25.54g/L、豆饼粉7.94 g/L、乙酸钠5.64 g/L;此条件下的验证试验表明,实际紫杉醇得率为313.52 μg/L,与理论值基本吻合。
    Abstract: Based on the results of single factor experiment, three levels and three factors of Box-Behnken design was employed combining with response surface methodology (RSM) to optimize the fermentation conditions for taxol production from endophytic fungus. The result showed that the optimal fermentation conditions were as follows: glucose 25.54 g/L, soybean powder7.94 g/L and sodium acetate 5.64g/L. Verification test demonstrated that the average yield of taxol was 313.52 μg/L, similar to theoretical value of 331.6 2μg/L.
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出版历程
  • 收稿日期:  2015-06-07
  • 修回日期:  2015-10-04
  • 刊出日期:  2015-12-29

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