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杜仲叶中绿原酸的提取方法与精制工艺研究

周爱存, 哀建国, 潘佳佳, 何斐

周爱存, 哀建国, 潘佳佳, 何斐. 杜仲叶中绿原酸的提取方法与精制工艺研究[J]. 浙江林业科技, 2016, 36(1): 42-46.
引用本文: 周爱存, 哀建国, 潘佳佳, 何斐. 杜仲叶中绿原酸的提取方法与精制工艺研究[J]. 浙江林业科技, 2016, 36(1): 42-46.
ZHOU Ai-cun, AI Jian-guo, PAN Jia-jia, HE Fei. Extracting Methods and Purification Technology of Chlorogenic Acid from Leaves of Eucommia ulmoides[J]. Journal of Zhejiang Forestry Science and Technology, 2016, 36(1): 42-46.
Citation: ZHOU Ai-cun, AI Jian-guo, PAN Jia-jia, HE Fei. Extracting Methods and Purification Technology of Chlorogenic Acid from Leaves of Eucommia ulmoides[J]. Journal of Zhejiang Forestry Science and Technology, 2016, 36(1): 42-46.

杜仲叶中绿原酸的提取方法与精制工艺研究

基金项目: 浙江省自然科学基金资助项目(Y3110393)
详细信息
    作者简介:

    周爱存(1984-),男,江苏宿迁人,硕士,讲师,从事中药材与种质资源开发研究。

  • 中图分类号: S789

Extracting Methods and Purification Technology of Chlorogenic Acid from Leaves of Eucommia ulmoides

  • 摘要: 对杜仲(Eucommia ulmoides)叶片中绿原酸的提取与精制工艺进行研究,比较了酸水提取、40%乙醇提取以及纤维素酶提取对绿原酸提取效果的影响,结果表明,酶提取更有利于后续工艺对绿原酸的精制。通过静态吸附试验,比较HPD-100A、AB-8、HPD-400A、DM-130、HPD-600、HPD-826六种大孔吸附树脂对绿原酸的吸附及脱附性能,筛选出性能较好的HPD-826树脂进行动态试验,结果表明,该树脂在室温下对杜仲叶中绿原酸的动态吸附-脱附最佳工艺参数为:上柱液pH值3.0,上柱流速2 BV/h,上柱体积7 BV;脱附剂为60%乙醇,洗脱流速1BV/h,洗脱剂用量为2 BV。用优化后的工艺进行试验,所得绿原酸粗产品的纯度可达49.85%,且工艺具有良好的稳定性。
    Abstract: Experiments were conducted on extracting of chlorogenic acid from leaves of Eucommia ulmoides by HCL solution, 40% ethanol and cellulose. The results showed that extracted solution by cellulose had better effect for the following purification. Comparison tests were implemented on adsorption and desorption of extracted solution of chlorogenic acid by HPD-100A, AB-8, HPD-400A, DM-130, HPD-600, HPD-826. HPD-826 had better properties and was selected for dynamic experiment. The result demonstrated that the optimal technology was as follows: 3.0 of sample pH, 2 BV/h of flow rate, 7 BV of bed volume, 60% alcohol as desorption agent, 1 BV/h of flow rate and 2 BV of desorption agent. Under the conditions above-mentioned, the purity of chlorogenic acid reached 49.85% , and the process stability was proved to be satisfactory.
  • [1] 刘颖,郭明晔,白根本. 绿原酸的研究进展[J]. 中药材,2012,35(7):1 180-1 185.
    [2] 哀建国,杜江丽,周爱存,等. 双波长HPLC同时测定氮沉降处理下杜仲皮和叶中的5种成分[J]. 浙江农林大学学报,2015,32(1): 60-66.
    [3] 宋宏新,戴瑜. 酶法提取杜仲叶中绿原酸工艺研究[J]. 西北植物学报,2006,26(11):2 383-2 387.
    [4] 尉芹,景谦平,马希汉. 杜仲叶中绿原酸的提取工艺条件研究[J]. 林产化学与产业,2001,21(4):27-32.
    [5] 王茜,李智,何琦,等. 杜仲叶中绿原酸提取分离工艺条件的研究[J]. 离子交换与吸附,2008,24(1):73-80.
    [6] 杨军宣,尹蓉莉,陈金玉,等. 金银花叶中绿原酸的大孔吸附树脂纯化工艺研究[J]. 中国实验方剂学杂志,2010,16(15):20-23.
    [7] 刘军海,裘爱泳. 杜仲叶绿原酸的提取及精制[J]. 山东医药,2004,44(32):7-9.
    [8] 熊伟,胡居吾,李雄辉,等. 大孔树脂分离纯化杜仲叶中绿原酸的研究[J]. 江西科学,2010,28(2):178-181.
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出版历程
  • 收稿日期:  2015-07-11
  • 修回日期:  2015-10-27
  • 刊出日期:  2016-06-29

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