Abstract: Carbonized reconstructed bamboo timber was treated by five anti-mildew and antiseptic agents such as ACQ (Ammoniacal CopperQuaternary), CuAz (Copper Triazole), ZJFC-I (Propiconazole and Tebuconazole), CCC (Chitosan Copper Complex), PINSKAN (Iodopropynylbutylcarbamate, Propiconazole and Tebuconazole) with three concentrations like 0.5%, 1.0% and 1.5% to test their antiseptic and antimildew capacitywith Penicillam citrinum, Trichoderma viride, Aspergillus nige, Poria placenta and Coriolus versicolor. The results showed that treated reconstructedbamboo timber had better antiseptic and antimildew. The drug loading had positive relation with agent concentrations, antiseptic and antimildewability. ZJFC-I and ACQ all had better antimildew abilities. 1.0% of ZJFC-I and 1.5% of ACQ had the best antimildew result, topped to 100%. 1.5%of CuAz had 81.25% and 87.50% effect against P. citrinum and T. viride. 1.5% of CCC and PINSKAN had 87.50% and 100% effect against A. niger..The antiseptid abilities of treated reconstructed bamboo timber increased by 60.4% with mean weight loss percentage less than 3.36%, and their decayresistance grade was level I. CuAz had best antibacterial effect with the same concentration of treatments. The control (no treatment) had not anymildew-proof capacity, but it had good antibacterial property with mean weight loss of 8.48%, and decay resistance grade was level I.