Abstract: Afforestation was carried out in February of 2015 at water-level fluctuation zone with different altitudinal gradient in Thousand IslandLake, Zhejiang province, with saplings about 3.2m high of Salix chienii, Taxodium ascendens, Metasequoia glyptostroboides, Liquidambarformosana, Pterocarya stenoptera, Populus x canadensis, Sapium sebiferum, Phyllostachys heteroclada. Complete enumeration was implemented inMarch of 2016. The result demonstrated that under dynamic water level fluctuation, survival rate of planted trees had great differences. S. chieniicould be resistant to water immersion and drowned water logging, survival rate still reached 70% after continuous drowned water logging 116 daysthroughout the year at 99m. T. ascendens and L. formosana could be resistant to water immersion and water logging, but not to be drowned water logging. Their survival rate reached 60% after continued drowned water logging 192 days at 102m, but died at 101m and below due to continuousdrowned of 21 days or more. M. glyptostroboides, P. stenoptera, P. ×canadensis and S. sebiferum was only resistant to wet, but not to high-levelwater logging and drowned. Their survival rate at 103m was respectively 20%, 60%, 80% and 40%. Recommendations were offered that afforestationby M. glyptostroboides, P. stenoptera and S. sebiferum seedlings should be 1m above the highest water level and 1.5m for P.×canadensis. Ph.heteroclada was resistant to water logging, but not to high-level water logging and drowned water logging, the survival rate at 104m was 40%, thedeepest drowned water logging depth was 0.52 m.