Abstract: Investigations were conducted on vine resources at different elevations in Tianmu Mountain National Reserve, Zhejiang province in August2016. Analysis was made on characters and application in urban landscape. The result demonstrated that there were 49 species, belonging to 24families and 41 genera. Distribution of vine species decreases with the increase of altitude. Quadrate survey showed that it had 17, 12, 10, 9 and 7species of vine at the altitudes of 300m, 470m, 620m, 870m and 1100m. Line survey indicated that there were 29, 13 and 8 vine species at the altitudeintervals of 300-500m, 500-800m and 800-1100m. Analysis on character of different vine species resulted that some of them could be planted in thecities for ground-covering, vertical greening, potted or courtyard greening.