Abstract: In June 2020, 3 typical quadrats were established in 4 research areas in nature growing Mangifera persiciformis in Nanning, Chongzuo and Bose of Guangxi. Soil bacterial community under Mangifera persiciformis was determined by high-throughput sequencing technology, and the soil chemical properties were measured. The results showed that there were 23 phyla, of which Proteobacteria and Acidobacteriota was dominant in the four research areas. A total of 118 genera were identified, of which Vicinamibacter, Pedosphaera and Nitrospira was dominant in the four areas. There were 123 species were identified, of which Vicinamibacter silvestris, Pedosphaera parvula, Gemmatimonas sp. and Terrimona sp. were dominant. Redundancy analysis demonstrated that the available potassium, available phosphorus and pH had great influence on the composition of bacterial phylum and genus levels, and the total phosphorus, available phosphorus, and soil pH had great influence on the composition of species level.