Abstract: Based on forest inventory data of 1995-2015 in Guizhou province, studies were made on spatial and temporal changes of forest carbon storage by biomass of volume source method. The results showed that the total carbon storage was obviously increased from 76.89 TgC in 1995 to 253.65 TgC in 2015 with an annual increment of 11.49%. The carbon density obviously increased from 23.57 Mg C/ha in 1995 to 32.59 Mg C/ha in 2015, resulted by large proportion of young and middle-aged forest. In Guizhou, forest carbon storage and density was higher in the northern and southeastern regions than that in the central and western regions. The spatial distribution pattern of forest carbon storage and density had no significant difference among different times.