Abstract: During July to September 2015, 2016, 2019, investigations and collections were conducted on macrofungi resources in Jingning Wangdongyang Alpine Wetland Nature Reserve (hereinafter referred to Reserve), Zhejiang province. Total of 435 samples were collected. The result demonstrated that there were 110 species, belonging to 68 genera, 39 families, 12 orders, 4 classes, and 2 phyla. Six species are new records in China, namely Lactarius austrorostratus, Psathyrella badhyzensis, L. formosus, Marasmiellus rhizomorphigenus, Xeromphalina kauffmanii and Entocybe trachyospora. Three families were rich in species, like Russulaceae, Agaricaceae, and Boletaceae, with a total of 33 species, accounting for 30% of the total species. Three genera were rich in species, like Amanita, Russula, and Entoloma, with a total of 21 species, accounting for 19% of the total ones. There were 38 species of woody fungi, 39 of soil fungi and 33 of mycorrhizal fungi. There were 22 species of edible mushrooms, 22 species of medicinal fungi, 33 species of ectomycorrhizal mushrooms and 20 species toxic mushrooms.