Abstract: In order to explore the influence of epispermon physiological and biochemical indexes during seed storage of Paris polyphylla, the changes of antioxidant enzyme activities, reactive oxygen species and endogenous hormone in seeds with and without episperm were studied during 0,4, 8 and 10 months of storage. The results showed that superoxide dismutase （SOD） and phenylalaninase （PAL） activities decreased during seed storage, and removing episperm could slow down the decrease of SOD and PAL activities. The POD activity of seed with episperm decreased, while that of seed without episperm increased. Before storage, the contents of auxin （indole acetic acid）, gibberellin 3 and cytokinin （kinetin, zeanoside, dihydrozein and trans zein） with episperm were significantly higher than those in seeds without seedcoat, while the contents of gibberellin 7,1-naphthalene, salicylic acid, jasmonic acid and abolic acid were significantly lower than those in seeds without episperm. With the extension of storage time, the contents of indole acetic acid in the seeds of the two treatments increased significantly, while the other hormones decreased significantly, indicating that indoleacetic acid, a growth hormone, may be one of the important endogenous hormones to promote seed germination. Therefore, seed storage by removing seed coat and indoleacetic acid treatment is beneficial to seed germination.