Identification of 12 Superior Cultivars of Camellia oleifera by Sequence CharacterizedAmplified Region Markers

Experiments were conducted on sequence characterized amplified region markers (SCAR) from amplified polymorphic DNA (SAPD),sequence-related amplified polymorphism and inter simple sequence repeat (ISSR) molecular techniques for identification of 12 cultivars (Changlinseries)of Camellia oleifera. The result demonstrated that twenty-one polymorphic bands (7 from nSAPD, 10 from SRAP and 4 from ISSR) wereobtained from the tested cultivars by using 43 primers (4 SAPD, 24 SRAP and 15 ISSR). Eleven (2 from nSAPD, 7 from SRAP and 2 from ISSR) of21 polymorphic bands were converted into 11 stable and reproducible SCAR markers by designing specific SCAR primers from the 11 sequencedpolymorphic bands and by verifying PCR amplification with SCAR primers. Changlin 23, 26, 53, 56 and 166 had only one SCAR marker, Changlin 3and 55 had two ones, Changlin 21 had four ones, Changlin 18 had six ones, and Changlin 4, 27 and 40 have no SCAR markers. These SCAR markerscan be used as cultivar-specific DNA fingerprints for better identification of cultivars.