Tissue Culture of Soot Apices for Induction of PLBs of Dendrobium nobile ‘Huoniao’

Experiments were conducted on induction, multiplication, differentiation and rooting of shoot apices of Dendrobium nobile ‘Huoniao’ asexplants treated by different plant growth regulators and basic medium. The results showed that the best basic medium and growth regulator was MSand 0.2 mg·L-1 of 2,4-D for induction of protocorm-like bodies(PLBs) with induction rate of 92%. 1.0 mg·L-1of NAA had the best effect ofproliferation of PLBs, with growth coefficient of 3.55 time. 0.5 mg·L-1 of NAA had the best effect of differentiation of PLBs. Addition of 5% coconutmilk and 1.0 g·L-1 activated carbon had advantages of rooting and seedling growth. The conclusions for the experiments were as follows, the bestcondition for induction, proliferation and differentiation was MS +2 mg·L-1 6-BA + 0.5 mg·L-1 NAA +0.2 mg·L-1 2,4-D, 1/2MS + 0.05 mg·L-1 KT +1.0 mg·L-1 NAA, and 1/2MS +0.5 mg·L-16-BA + 0.5 mg·L-1 NAA. The survival rate of in-vitro plantlets was 86% under 25℃ and 1 500 Lx lightintensity in light incubator.