SRAP Analysis on Genetic Relationship of Germplasm Resources of Ficus carica in Zhejiang Province

Fifteen culitvars of Ficus carica from the United States, the United Kingdom, Italy, France and China were planted in Wuyi, Zhejiangprovince in 2015, leaves were collected in March 2016 for evaluation of genetic relationships among these cultivars by SRAP markers andobservations on phenological periods. The results showed that there were differences in phenological period among 15 germplasm resources, withsprouting period from middle to late March, leaf expansion from middle to late April, fruit setting at early May, and fruit mature from late June to theearly September. 34 SRAP primers were selected, 204 polymorphic bands were obtained by PCR with the polymorphic rate of 62.25%, indicatingabundant intraspecific variation. The genetic similarity coefficient among 15 cultivars was 0.691-0.922, with the maximal coefficient of 0.922 of‘A132’ and ‘Masui Dauphine’, and the minimal of 0.691 of ‘Violette Solise’ and ‘Hongaisheng’. Cluster analysis showed that tested germplasm couldbe divided into two groups at genetic distance of 0.735. ‘Hongaisheng’ was clustered alone, the others were clustered into one group. Observation onphenological period of tested cultivars planted in the same location showed that their phenology had relation with their provenances..