Optimizing Technology for Ultrasonic Extraction of Total Flavonoidsfrom Inonotus sanghuang
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Abstract
Extraction technology of flavonoids from Inonotus sanghuang was optimized by ultrasonic-assisted method. Based on the results ofone-factor-at-a-time-technique method, a three-level Box-Behnken factorial design was implemented combining with response surfacemethodologyto optimize the extraction conditions of total flavonoids. The result demonstrated that theoretical yield of flvonoids could reach 2.63%underultrasonic temperature of 55.2℃, ethanol concentration of 70.9% and ultrasound treatment time of 47.8minutes. Verification test resulted theaverage yield of 2.51%, similar to theoretical value.
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