Morphology and Identification of Truffles in the Soil of Pinus sylvestris var. mongolica Forest

In May and October of 2014 and 2015, collections of soil sample were implemented under Pinus sylvestris var. mongolica forests in 6 forest farms in the Nei Monggol Autonomous Region. Roots with mycorrhiza were selected from soil sample and two types of truffle were described and identified. The extraction of genomic DNA of two truffles by CTAB method, and on the basis of PCR, ITS regions of DNA of the macrofungi were amplified and sequenced by using the special pairs of primers ITS1F/ITS4. Their sequences were compared with those registered in GenBank database. They were belonged to Tuber, and one of them was T. liaotongense。