Cloning and Bioinformatics Analysis of Bxpel1 Gene from Bursaphelenchus xylophilus

Bxpel1 gene sequence from Bursaphelenchus xylophilus cDNA as a template was amplified and cloned by RT-PCR. Bxpel1 gene (795bp) was successfully cloned with GC content of 50.44% and encode of 252 amino acids. The cloned sequence had 98% similarity to the pel1 gene previously published in the GenBank (ID: AB232908.1). Phylogenetic analysis showed that Bxpel1 had high homologies with that from B. mucronatus and Aphelenchus avenae. Bxpel1 gene encode from 0 to 25 sequences may be transmembrane structure, while the other sequences outside membrane. The secondary and tertiary structures were abundant in random coils and beta helix. The signal peptide cleavage sites locate between No. 17 to No. 18 amino acid and performs biological effects at extracellular.