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QIN Yu-chuan, WANG Li-ling, WANG Yan-bin, HE Liang, LIU Ben-tong, QIAN Hua, TONG Xiao-qing. Purification and Analysis of DWW-1 from Dendrobium wardianum[J]. Journal of Zhejiang Forestry Science and Technology, 2018, 38(4): 15-20. DOI: 10.3969/j.issn.1001-3776.2018.04.003
Citation: QIN Yu-chuan, WANG Li-ling, WANG Yan-bin, HE Liang, LIU Ben-tong, QIAN Hua, TONG Xiao-qing. Purification and Analysis of DWW-1 from Dendrobium wardianum[J]. Journal of Zhejiang Forestry Science and Technology, 2018, 38(4): 15-20. DOI: 10.3969/j.issn.1001-3776.2018.04.003
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Purification and Analysis of DWW-1 from Dendrobium wardianum

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  • Crude polysaccharides from Dendrobium wardianum were extracted by water and precipitated by ethanol. Deproteinization with Sevage method, DWW-1 was separated and purified by DEAE-Cellulose and Sephadex G-75 column chromatography. Analysis of properties and structure was performed by using chemical method, HPGPC, HPLC, GC-MS and IR. The results showed that DWW-1 was a white flocculent solid after vacuum freeze-drying, soluble in water, with relative molecular mass of 6.479×104. It was composed of glucose, glucuronic acid, mannose and fucose. DWW-1 showed strong scavenging ability on DPPH free radicals.
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