Preliminary Test on Establishment of Sterile Culture of Taxodium ‘Zhongshanshan’

Current spring and autumn shoots of 8-year Taxodium ‘Zhongshanshan’ were collect on 16 November of 2017 at cutting orchard in Chongqing for test of sterile culture of explant and axillary bud germination.Sterile culture test was designed by randomized block of three factors, namely type of shoot, concentration and treatment time of HgCl2.The results showed that the best sterile culture of explant wasautumn shoot treated by 0.2% HgCl2 for 10 minutes, withcontaminationrate of 0.Axillary bud germination test was designed by orthogonal of L9 (34), with NAA, 6-BA and activated carbon as factors.The results indicated that NAA and 6-BA had the positive effect ongermination of axillary bud, especially 6-BA, while activated carbon had inhibitory effect.The optimal medium of bud germination was MS + 1.0mg?L-1of 6-BA + 0.5mg?L-1NAA.