Isolation and Identification of Mycorrhizal fungi in Rhododendron huadingense Root in Zhejiang Dapanshan National Nature Reserve

Three sample plots were established in Dapanshan National Nature Reserve, Zhejiang province in May of 2016. 5 Rhododendron huadingense were selected for 1-2 lateral roots each. Ericoid mycorrhiza (ERM) was cultured from collected roots and studied by morphological characteristics and molecular identification of rDNA ITS sequence. The result showed that 165 mycorrhizal fungal strains were isolated which were classified into 22 types based on colony characteristics. rDNA ITS demonstrated that these mycorrhizal fungi could be identified into 20 species, among them, 19 species belonging to Ascomycota, including 8 Sordariomycetes, 6 Leotiomycetes. The dominant 3 species were Phialocephala fortinii, Ilyonectria radicicola and Sordariomycetes sp., respectively accounting for 41.82 %, 22.42% and 10.91% of the total strains. The lest 17 species occupied only 24.9%.