Inhibition Effect of Extracts from Cyclocarya paliurus Leaf on Activity of Disaccharide in Caco-2 cells

Collection was carried out of leaves of 5-year Cyclocarya paliurus planted in Suichang, Zhejiang province by the end of September 2016. Experiments were implemented on effect of water extract, water extract after alcohol extraction, 95% alcohol extract, ethyl acetate extract and n-butyl alcohol extract from leaves of C. paliurus on cell activity of caco-2 cells and inhibitory effects on activity of maltase, sucrose and lactase. The results showed that 95% alcohol extract had impact on the activity of caco-2 cells and the other four extracts had non-toxic to caco-2 cells. Water extract after alcohol extraction had the best inhibitory effect on the activity of disaccharide, especially on that of sucrose and lactase, with the highest inhibition rate of 76.35% and 60.13%, followed by ethyl acetate extract, with the highest inhibition rate of lactase of 54.41%.