Effect of H2S on Sulfur and Nitrogen Metabolism in Cell Suspension Culture of Betula platyphylla

Experiments were carried out on effect of H2S treatment on sulfur metabolism and nitrogen metabolism in cell suspension culture of Betula platyphylla.Sterile hydrogen sulfide (H2S) solutions of 0 (CK), 0.5, 1.0 and 5.0 mmol?L-1 were respectively added to the suspension culture of 8 days, and during 0 to 5 days of treatment, pH value, electrical conductivity, malondialdehyde (MDA), superoxide anion content, sulfur and nitrogen metabolism index were determined every day.The results showed that it had different effect of different NaHS concentration and duration.5 mmol?L-1 NaHS treatment had increase of above-mentioned index.The activity of ATPS, GSH content, nitrate nitrogen and ammonia nitrogen content, NR and GS activity were increased by different concentrations of NaHS treatment.The experiment demonstrated that NaHS treatment had the greatest influence on ammonia nitrogen content.