Identification of Species of Taxus by DNA Barcoding Based on ITS Sequence
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YIN Wen-xiu,
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ZHANG Ming-zhe,
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LOU Cheng-jie,
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WANG Jin-zhuan,
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YU Wen-tao,
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HU Mei-ling,
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WU Shan,
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YU Hui-zhen,
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XU Jin,
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ZHANG Xiao-feng,
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LI Ming-fu
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Abstract
Fresh branches of Taxus were collected from 8 trees in Shanghai, Zhejiang and Jiangsu in March 2017. They were PCR amplified and sequenced by DNA barcoding, and the results were compared with the homologous sequences in GenBank. The results showed that 4 samples had the highest sequence similarity with T. wallichiana var. mairei. Two samples had the highest sequence similarity with T. cuspidata, and the other 2 samples with T. baccata. Phylogenetic tree established by neighbor-joining method based on ITS1-18S sequences indicated that 4 samples gathered with T. wallichiana var. mairei , 2 samples with T. cuspidat, and 2 samples with T. baccata. Analysis resulted that 4 samples were T. wallichiana var. mairei, 2 samples T. cuspidate and 2 samples T. baccata, which were completely consistent with the actual sampling tree species.
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