In vitro Propagation of Wikstroemia monnula from Stem and Leaf

Tissue culture of current year leaf and stem of Wikstroemia monnula was conducted in Hangzhou, Zhejiang province. Experiments were carried out on different explant disinfections, medium for induction, differentiation, multiplication and cultivation. The results showed that the best disinfection method was to soak the explants in 0.25% sodium hypochlorite solution for 10 minutes. Induction medium of WPM+0.01 mg/L of 6-BA could introduce bud from 45.3% of the stem. MS+4 mg/L of TDZ+2 mg/L of 2,4-D had the highest callus induction ratio of 65.1%, and then MS+2.5 mg/L of TDZ+1.0 mg/L of NAA had bud induction of 64.9%. WPM+0.1 mg/L of 6-BA+0.1 mg/L of NAA had multiplication coefficient of 4.84, while WPM+0.1 mg/L of 6-BA+0. mg/L of NAA+1 000 mg/L of PVP could increase multiplication coefficient to 5.42.