Genetic Relationship of Polygonatum kingianum with Three Different Flower Colors

In July 2019, Polygonatum kingianum with flower of purple safflower, white flowers and purple spots on white flowers were collected 48 individuals each in Dali, Yunnan province for ISSR molecular marker analysis. The results showed that a total of 103 bands were obtained from 48 P. kingianum individuals amplified with 11 ISSR primers, among which, 99 bands were polymorphic, and the genetic identity was 0.89-0.91. The genetic difference and UPGMA clustering analysis showed that the white flower genotype (P1), the white flower with purple spot genotype (P2) and the purple safflower genotype (P3) could be grouped into one group, among which, there was closer genetic relationship between P2 and P3.Therefore, the P2 might be the transitional genotype between P3 and P1, or a heterozygous state of the offspring produced by the plants of the white flower genotypes after receiving the pollen of the purple safflower.