Culture of Peripheral Lymphocyte and Chromosome Analysis of Panthera tigris amoyensis

Human peripheral blood lymphocyte culture medium was used to culture the peripheral Homo sapiens blood lymphocytes of Panthera tigris amoyensis, then the cultured cells fixed, the chromosomes spread, and G-banding by trypsin digestion. The results showed that there were 38 chromosomes, its karyotype formula was: 6 (m) +6 (sm) +3 (st) +2 (t), X, Y (m, m), of which the 12th pair was the satellite chromosome. G-banding characteristics P. tigris amoyensis chromosomes could be divided into 6 types, including 6 types A, 8 types B, 4 types C, 8 types D, 6 types E, 4 types F, and sex chromosomes X and Y. However, the long arm of D3 chromosome of P. tigris amoyensis lacks a hyper chromatic band, and monomeric heteromorphic occurs on C1 and F2 chromosomes.