Embryo Culture in Vitro and Rapid Propagation Technology of Viburnum taitoense ×Viburnum suspensum

In order to shorten the breeding process of Viburnum plants, mature embryos of Viburnum taitoense × Viburnum suspensum were used as explants for tissue culture and rapid propagation research. The results showed that the combined treatment of 15 seconds’ 70% ethanol disinfection followed by 0.1% mercuric chloride for 25 min was the most effective for sterilization of V. taitoense × V. suspensum seeds, which not only ensured a high germination rate, but also controlled the pollution rate below 7%. The optimal medium for the start-up culture was 1/2 MS + 0.3 g·L^-1 lactalbumin hydrolysate, achieving a germination rate of 91.95%. The best multiplication culture medium was 1/2MS+0.5 mg·L^-16-BA+0.2 g·L^-1 IBA+0.3 g·L^-1lactalbumin hydrolysate, yielding a multiplication coefficient of 4.83. The optimal rooting culture medium was 1/2 MS + 0.5 mg·L^-1 IBA+ 0.05 mg·L^-1 NAA, resulting in a rooting rate of 90.28%. In this study, the in vitro culture and rapid propagation technology system of distant hybrid embryos of Viburnum is established. This system greatly promotes the efficiency of hybrid breeding of Viburnum species and accelerates the breeding process, and also provides a valuable reference for research on embryo culture of other woody ornamental plants.