Storage Substance Content and Their Enzyme Activity During Stratification of Torreya grandis Seed

Seeds of Torreya grandis were collected in the Oct., 2013 in Shengzhou, Zhejiang province, and were stratified on the same month. Determinations were made on content of starch, soluble sugar, protein, fat, activities of amylase, protease, acid phosphatase. The results demonstrated that 150 days later, the content of starch, protein and crude fat of treated seeds decreased by 40.30%, 57.78% and 22.45% respectively, while the content of soluble sugar decreased first and then increased. The activity of amylase, protease and acid phosphatase increased from 0 to 120th days, and slightly decreased from 120th to 150th days of the stratification. The experiment indicated that the storage substances decomposed under the catalysis of relative enzymes for providing energy of embryo development and seed germination