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CHENG Jun-wen, HE Liang, ZOU Jing-quan, FANG Ru, QIU De-you, HU Chuan-jiu, WEI Hai-long, JIANG Yun-he, WANG Yan-bin. Optimization of Fermentation Conditions for Taxol Production from Endophytic Fungus by Response Surface Methodology[J]. Journal of Zhejiang Forestry Science and Technology, 2015, 35(6): 68-72.
Citation: CHENG Jun-wen, HE Liang, ZOU Jing-quan, FANG Ru, QIU De-you, HU Chuan-jiu, WEI Hai-long, JIANG Yun-he, WANG Yan-bin. Optimization of Fermentation Conditions for Taxol Production from Endophytic Fungus by Response Surface Methodology[J]. Journal of Zhejiang Forestry Science and Technology, 2015, 35(6): 68-72.
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Optimization of Fermentation Conditions for Taxol Production from Endophytic Fungus by Response Surface Methodology

  • 1.

    Key Laboratory of Forest Food of Zhjiang, Zhejiang Forestry Academy, Hangzhou 310023, China

  • 2.

    State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

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  • Received Date: June 07, 2015
  • Revised Date: October 04, 2015
    Graphical Abstract
    • Abstract
  • Based on the results of single factor experiment, three levels and three factors of Box-Behnken design was employed combining with response surface methodology (RSM) to optimize the fermentation conditions for taxol production from endophytic fungus. The result showed that the optimal fermentation conditions were as follows: glucose 25.54 g/L, soybean powder7.94 g/L and sodium acetate 5.64g/L. Verification test demonstrated that the average yield of taxol was 313.52 μg/L, similar to theoretical value of 331.6 2μg/L.
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    • References (9)
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