Identification and Genetic Diversity of Cultivated <i>Morchella</i> spp.

LI Yingping; WEI Hailong; ZHOU Xinyi; YANG Chunbo; HU Chuanjiu

doi:10.3969/j.issn.1001-3776.2024.03.007

Journal of Zhejiang Forestry Science and Technology > 2024 > 44(3): 49-59. > DOI: 10.3969/j.issn.1001-3776.2024.03.007

LI Yingping, WEI Hailong, ZHOU Xinyi, YANG Chunbo, HU Chuanjiu. Identification and Genetic Diversity of Cultivated Morchella spp.[J]. Journal of Zhejiang Forestry Science and Technology, 2024, 44(3): 49-59. DOI: 10.3969/j.issn.1001-3776.2024.03.007

Citation:

PDF (984 KB)

Identification and Genetic Diversity of Cultivated Morchella spp.

LI Yingping^{1, 2,},
WEI Hailong^2, ,,
ZHOU Xinyi^{1, 2},
YANG Chunbo^{1, 2},
HU Chuanjiu²

1.
College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China
2.
Zhejiang Academy of Forestry, Hangzhou 310023, China

More Information

Received Date: February 09, 2024
Revised Date: April 17, 2024
Available Online: July 04, 2024

Graphical Abstract

Abstract

Abstract

41 cultivated cultivated Morchella spp. germplasms were bought from domestic markets of 7 provinces of China. Identification was carried out by multi gene joint matrix sequences, and genetic diversity was analyzed by ISSR molecular marker with morphological indicators. The results showed that phylogenetic tree based on multiple gene sequences clustered 41 strains into two branches, 39 strains from different provinces clustered into one branch with M. sextellata, and the remaining 2 strains clustered with M. importuna. Ten ISSR primers amplified 62 bands, with polymorphism ratio of 85.48%. The number of effective alleles (Ne) was 1.372 2, the gene diversity index was 0.241 3, and the Shannon's information index was 0.386 7. There was no clear correlation in genetic differentiation coefficient, genetic distance among different provinces. Population structure analysis and principal component analysis also showed that the correlation with geographical location was not significant. Principal component analysis demonstrated the same relation. 37 stains of the total 41 ones had a Q value ≥ 0.6, indicating that most of the tested strains had a relatively single kinship. Based on phenotypic traits and ISSR clustering, the test strains could be divided into 5 groups, and there were a litter differences in the clustering results between morphological markers and ISSR markers in some stains.41 cultivated Morchella spp. germplasms were bought from domestic markets of 7 provinces of China. Identification was carried out by multi gene joint matrix sequences, and genetic diversity was analyzed by ISSR molecular marker with morphological indicators. The results showed that phylogenetic tree based on multiple gene sequences clustered 41 strains into two branches, 39 strains from different provinces clustered into one branch with M. sextellata, and the remaining 2 strains clustered with M. importuna. Ten ISSR primers amplified 62 bands, with polymorphism ratio of 85.48%. The number of effective alleles (Ne) was 1.372 2, the gene diversity index was 0.241 3, and the Shannon's information index was 0.386 7. There was no clear correlation in genetic differentiation coefficient, genetic distance among different provinces. Population structure analysis and principal component analysis also showed that the correlation with geographical location was not significant. Principal component analysis demonstrated the same relation. 37 stains of the total 41 ones had a Q value ≥ 0.6, indicating that most of the tested strains had a relatively single kinship. Based on phenotypic traits and ISSR clustering, the test strains could be divided into 5 groups, and there were a litter differences in the clustering results between morphological markers and ISSR markers in some stains.
- Morchella,
- Multigene,
- Genetic diversity,
- Morphological markers,
- population structure

FullText(HTML)

References (28)

References

[1]	杜习慧,赵琪,杨祝良. 羊肚菌的多样性、演化历史及栽培研究进展[J]. 菌物学报,2014,33(2):183 − 197.
[2]	赵曜,张强. 羊肚菌的营养成分和药理作用研究进展[J]. 中国林副特产,2022(1):71 − 74.
[3]	田金凤,尚远宏,肖宗妮. 羊肚菌的营养成分、功能和加工的研究进展[J]. 食品工业科技,2023(1):1 − 20.
[4]	么越,荣丹,唐梦瑜,等. 羊肚菌药用价值及产品开发现状[J]. 中国食用菌,2022,41(7):13 − 17,21.
[5]	杨璐敏,熊永生,华蓉,等. 我国羊肚菌产业发展的SWOT分析[J]. 中国食用菌,2022,41(12):57 − 62,72.
[6]	TA K H,BUYUKALACA S,DOGAN H H,et al. A multigene molecular phylogenetic assessment of true morels (Morchella) in Turkey[J]. Fungal Genet Biol,2010,47(8):672 − 682. doi: 10.1016/j.fgb.2010.05.004
[7]	武冬梅,李先义,高能,等. 采用多基因联合方法鉴定新疆野生羊肚菌[J]. 分子植物育种,2022,20(7):2420 − 2427.
[8]	杜军华,姜东伯,张津京,等. 青藏高原东部22株栽培与野生羊肚菌的分子进化分析[J]. 江苏农业科学,2022,50(7):28 − 34.
[9]	闫晓雪,时小东,张国珍,等. 西南地区部分野生羊肚菌ITS序列鉴定及生境分析[J]. 微生物学杂志,2016,36(6):48 − 53. doi: 10.3969/j.issn.1005-7021.2016.06.008
[10]	孟虎,孙国琴,睢韡,等. ISSR技术在食用菌研究上的应用[J]. 北方园艺,2016(5):207 − 210.
[11]	刘文丛,张建博,桂明英,等. 滇西北地区5种羊肚菌遗传多样性的ISSR分析[J]. 中国食用菌,2011,30(4):38 − 42. doi: 10.3969/j.issn.1003-8310.2011.04.011
[12]	于凤明,赵琪. 羊肚菌不同单孢及不同萌发孔菌丝间的遗传多样性[J]. 菌物学报,2020,39(6):1117 − 1129.
[13]	王红,曹君,张敏,等. 辽宁省野生羊肚菌资源调查与遗传多样性分析[J]. 中国食用菌,2021,40(2):54 − 61.
[14]	吕锐玲,陈实,王杰,等. 基于ISSR标记的我国羊肚菌栽培品种遗传多样性分析[J]. 北方园艺,2023(8):107 − 11.
[15]	陈影,唐杰,彭卫红,等. 梯棱羊肚菌种质资源数量性状变异及概率分级[J]. 食用菌学报,2017,24(1):27 − 32.
[16]	曹雪莲,陈影,彭卫红,等. 毛头鬼伞种质资源农艺性状遗传多样性分析及综合评价[J]. 食用菌学报,2022,29(5):53 − 64.
[17]	徐志婷,许玲玲,薛龙海. 基于ISSR标记的多花黑麦草附球菌遗传多样性分析[J]. 西南农业学报,2023,36(9):1934 − 1941.
[18]	沈颖越,金群力,蔡为明,等. 基于重测序信息的金针菇菌株资源遗传多样性及群体结构分析[J]. 菌物学报,2020,39(6):1016 − 1028.
[19]	沈秀芬,章炉军,张美彦,等. 利用InDel标记分析中国香菇菌株的遗传多样性与群体结构[J]. 菌物学报,2021,40(9):2266 − 2281.
[20]	李赢,刘海翠,石吕,等. 江苏裸大麦种质资源遗传多样性和群体结构分析[J]. 作物学报,2023,49(10):2687 − 2705.
[21]	FALUSH D,WIRTH T,LIN Z B,et al. Traces of human migrations in Helicobacter pylori popul ations[J]. Science,2003,299:1582 − 1585.
[22]	刘伟,兰阿峰,张倩倩,等. 羊肚菌栽培菌株遗传多样性分析及种特异性RAPD-SCAR标记开发[J]. 菌物学报,2018,37(12):1650 − 166.
[23]	刘亚群,王燕飞,段柳会,等. 浙江省无花果种质资源亲缘关系的SRAP分析评价[J]. 浙江林业科技,2017,37(5):23 − 28. doi: 10.3969/j.issn.1001-3776.2017.05.004
[24]	SINGH P,JAISWAL J P. Assessing genetic diversity in bread wheat using inter simple sequence repeat (ISSR) markers[J]. Asian J Bio Sci,2016,11(1):141 − 145. doi: 10.15740/HAS/AJBS/11.1/141-145
[25]	杨燕,田鸿,张小平,等. 基于ITS和ISSR的羊肚菌种质资源遗传多样性分析[J]. 西南农业学报,2018,31(10):2004 − 2009.
[26]	TAYLOR J W,JACOBSON D J,KROKEN S,et al. Phylogenetic species recognition and species concepts in fungi[J]. Fungal Genet Biol,2000,31(1):21 − 32.
[27]	陆欢,尚晓冬,宋春艳,等. 基于ISSR标记和农艺性状的金顶侧耳种质资源遗传多样性分析[J]. 食用菌学报,2022,29(6):14 − 24.
[28]	吴小燕,鲍红春,李小雷,等. 26个香菇菌株的遗传多样性ISSR分析[J]. 种子,2023,42(5):63 − 67,85.

[1]	LI Dongbin, XU Jing, XU Zhibin, HE Liping, WEI Jingjing, LI Xiupeng. Natural Regeneration Seedlings of Cultivated Metasequoia glyptostroboides and Population Structure[J]. Journal of Zhejiang Forestry Science and Technology, 2024, 44(4): 7-13. DOI: 10.3969/j.issn.1001-3776.2024.04.002
[2]	YU Min-fen, MEI Jia-qi, HE Li-ping, LI Dong-bin, XU Dong-bin, YANG Ying, YUAN Hu-wei, YAN Dao-liang, ZHENG Bing-song. Genetic Diversities and Relationships of 9 Introduced Species/Cultivars of Quercus by ISSR Molecular Markers[J]. Journal of Zhejiang Forestry Science and Technology, 2022, 42(5): 9-15. DOI: 10.3969/j.issn.1001-3776.2022.05.002
[3]	QIAN Hai-yuan, YU Jian-ping, FAN Qing-ping, SONG Xiao-you, CHEN Sheng-wen, CHEN Jian-hua, YE Duo. Population Structure and Distribution of Camellia chekiangoleosa in Gutianshan NationalNature Reserve[J]. Journal of Zhejiang Forestry Science and Technology, 2020, 40(6): 46-51. DOI: 10.3969/j.issn.1001-3776.2020.06.007
[4]	JIANG Shun-bang, YUAN Cong-jun, YU De-hui, DAI Xiao-yong, YANG Bing, LI He, LONG Hai-yan. Population Structure and Dynamics of Rhododendron feddei[J]. Journal of Zhejiang Forestry Science and Technology, 2020, 40(4): 1-9. DOI: 10.3969/j.issn.1001-3776.2020.04.001
[5]	CHEN Jiang-fang, ZHOU Yu-hong, XU Xiao-feng, WANG Pan, ZHANG Su-jiong, Kang Hua-jing. Community Characteristics and Population Structure of Hamamelismollis Communities in Gaomu Mountain[J]. Journal of Zhejiang Forestry Science and Technology, 2018, 38(6): 8-13. DOI: 10.3969/j.issn.1001-3776.2018.06.002
[6]	JIANG Dong-yue, SHEN Xin, WU Fan, LI Yin-gang, SHI Cong-guang, YANG Shao-zong, LIU Xin-hong. Genetic Diversities of Cerasus spp. from Zhejiang Province Based on ISSR Markers[J]. Journal of Zhejiang Forestry Science and Technology, 2018, 38(4): 1-7. DOI: 10.3969/j.issn.1001-3776.2018.04.001
[7]	CHEN Xiao-xi, SHEN Chang-qing, HONG Wen-jun, ZENG Si-jin, ZHOU Yu-jie, HUANG Jiu-xiang. Community Structure and Species Diversity of Loropetalum subcordatum in MaolanNational Nature Reserve of Guizhou[J]. Journal of Zhejiang Forestry Science and Technology, 2017, 37(6): 22-28. DOI: 10.3969/j.issn.1001-3776.2017.06.004
[8]	XIA Xiao-mei, WANG Juan, PENG Pei-hao, LIU Xian-an. Relationship of Population Structure of Paeonia szechuanica with EnvironmentalFactors in Sichuan Province[J]. Journal of Zhejiang Forestry Science and Technology, 2017, 37(2): 30-35. DOI: 10.3969/j.issn.1001-3776.2017.02.005
[9]	SHENG Wei-xing, WU Chu-ping, FANG Yao-yao, CAI Jian-rong, JIANG Jian-chao, HUANG Yu-jie, HU Fei, YUAN Wei-gao. Investigation and Analysis on Community Structure of Natural Forest in Jiande[J]. Journal of Zhejiang Forestry Science and Technology, 2017, 37(2): 24-29. DOI: 10.3969/j.issn.1001-3776.2017.02.004
[10]	LI Gui-xiang, CHAI Yong, SHAO Jin-ping, WANG Yuan-zhong, BI Bo, HE Li-ping, MENG Guang-tao, FANG Bo. Community Structure of Rhododendron protistum var. giganteum in Gaoligong Mountain[J]. Journal of Zhejiang Forestry Science and Technology, 2016, 36(3): 1-7.

Cited By

Cited by

Periodical cited type(6)

1.	张忠钊，陈锋，谢文远，叶文国，陈征海. 浙江东部蔷薇科一新种——华顶悬钩子. 杭州师范大学学报(自然科学版). 2023(06): 611-615 .
2.	郑子洪，谢文远，刘菊莲，潘成椿，张方钢，陈征海. 浙江蔷薇科一新种——九龙山悬钩子. 杭州师范大学学报(自然科学版). 2022(06): 595-598+630 .
3.	陈高坤，张方钢，谢文远，陈锋，陈征海. 浙江蔷薇科悬钩子属一新种——陈谋悬钩子. 温州大学学报(自然科学版). 2021(01): 59-62 .
4.	雷祖培，刘菊莲，陈林，张方钢，谢文远，陈征海. 浙江蔷薇科植物新资料. 杭州师范大学学报(自然科学版). 2021(03): 274-279 .
5.	赵昌高，徐洪峰，张方钢，陈锋，谢文远，陈征海. 浙江蔷薇科悬钩子属一新种——景宁悬钩子. 杭州师范大学学报(自然科学版). 2020(03): 244-246+316 .
6.	徐洪峰，陈林，张方钢，梅旭东，谢文远，陈征海. 浙江悬钩子属（蔷薇科）植物新资料（Ⅱ）. 浙江林业科技. 2020(03): 85-90 . 本站查看

Other cited types(0)

Get Citation

PDF

XML

Article views (32) PDF downloads (9) Cited by(6)

Turn off MathJax

Article Contents

Abstract

References

Identification and Genetic Diversity of Cultivated Morchella spp.