Determination of Pigment Content in Different Cultivars of Malus spp. Leaves at Different Position

Five cultivars of Malus spp. （namely ‘Winter Red’， ‘Addirondack’， ‘Adams’， ‘Rudolph’ and ‘Perfect Purple’） with different color in leaf were selected from a total of 34 cultivars for measuring their pigment content at upper， middle and lower leaf position by spectral reflectance and solution method. Absolute and relative contents of chlorophyll， carotenoids and anthocyanins of determined cultivars were coupled function to calculate absolute pigment content in leaves of the left 29 cultivars. The result demonstrated that correlation coefficient R2 was 0.9875 for chlorophyll， 0.9994 for carotenoids and 0.9905 for anthocyanins. Chlorophyll content in leaves at upper position was lower than that at middle and lower position， but carotenoids and anthocyanins content was the opposite.