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SHEN Shao-yan, XIE De-jin, WU Yu-xiang, RONG Jun-dong, HE Tian-you, CHEN Li-guang, ZHENG Yu-shan. Comparison on Methods of Total RNA Extraction fromSarcandra glabra Leaves[J]. Journal of Zhejiang Forestry Science and Technology, 2016, 36(5): 40-44.
Citation: SHEN Shao-yan, XIE De-jin, WU Yu-xiang, RONG Jun-dong, HE Tian-you, CHEN Li-guang, ZHENG Yu-shan. Comparison on Methods of Total RNA Extraction fromSarcandra glabra Leaves[J]. Journal of Zhejiang Forestry Science and Technology, 2016, 36(5): 40-44.
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Comparison on Methods of Total RNA Extraction fromSarcandra glabra Leaves

  • 1.

    Department of Landscape,Fujian Agriculture and Forestry University,Fuzhou 350002,China

  • 2.

    Department of forestry, Fujian Agriculture andForestry University, Fuzhou, 350002,China

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  • Received Date: May 08, 2016
  • Revised Date: July 22, 2016
    Graphical Abstract
    • Abstract
  • Experiments were conducted on total RNA extraction from Sarcandra glabra leaves by CTAB, CTAB-LiCl, CTAB-Trizol, Trizol,TIANGEN RNAprep Pure Plant Kit and Bio Teke RNApure Total RNA Plant kit. Comparisons were made on the extraction efficiencies by UVspectrophotometer detection and electrophoresis. The result showed that extraction by CTAB-LiCl and TIANGEN RNAprep Pure Plant Kit wasbetter for total RNA in leaves of S. glabra, because the total RNA extracted by above two methods had better integrity and purity without obviouscontamination like proteins and other impurities. The ratio of OD260/OD280 was among 1.9-2.1, OD260/OD230 among 2.0-2.4.
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    • References (21)
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